Characterization of an enhanced antigenic change in the pandemic 2009 H1N1 influenza virus haemagglutinin.
Identifieur interne : 000231 ( Main/Exploration ); précédent : 000230; suivant : 000232Characterization of an enhanced antigenic change in the pandemic 2009 H1N1 influenza virus haemagglutinin.
Auteurs : Blanca García-Barreno [Espagne] ; Teresa Delgado [Espagne] ; Sonia Benito [Espagne] ; Inmaculada Casas [Espagne] ; Francisco Pozo [Espagne] ; María Teresa Cuevas [Espagne] ; Vicente Mas [Espagne] ; Alfonsina Trento [Espagne] ; Ariel Rodriguez-Frandsen [Espagne] ; Ana Falc N [Espagne] ; Juan Ortín [Espagne] ; Amelia Nieto [Espagne] ; José A. Melero [Espagne]Source :
- The Journal of general virology [ 1465-2099 ] ; 2014.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Analyse de séquence d'ADN (MeSH), Animaux (MeSH), Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Antigènes viraux (génétique), Antigènes viraux (immunologie), Données de séquences moléculaires (MeSH), Espagne (MeSH), Glycoprotéine hémagglutinine du virus influenza (génétique), Glycoprotéine hémagglutinine du virus influenza (immunologie), Grippe humaine (virologie), Humains (MeSH), Mutation faux-sens (MeSH), Protéines mutantes (génétique), Protéines mutantes (immunologie), Souris (MeSH), Souris de lignée BALB C (MeSH), Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H1N1 du virus de la grippe A (isolement et purification), Épitopes (génétique), Épitopes (immunologie).
- MESH :
- génétique : ARN viral, Antigènes viraux, Glycoprotéine hémagglutinine du virus influenza, Protéines mutantes, Sous-type H1N1 du virus de la grippe A, Épitopes.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Antigènes viraux, Glycoprotéine hémagglutinine du virus influenza, Protéines mutantes, Sous-type H1N1 du virus de la grippe A, Épitopes.
- isolement et purification : Sous-type H1N1 du virus de la grippe A.
- virologie : Grippe humaine.
- Analyse de séquence d'ADN, Animaux, Données de séquences moléculaires, Espagne, Humains, Mutation faux-sens, Souris, Souris de lignée BALB C.
- Wicri :
- geographic : Espagne.
English descriptors
- KwdEn :
- Animals (MeSH), Antibodies, Monoclonal (immunology), Antibodies, Viral (immunology), Antigens, Viral (genetics), Antigens, Viral (immunology), Epitopes (genetics), Epitopes (immunology), Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans (MeSH), Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza, Human (virology), Mice (MeSH), Mice, Inbred BALB C (MeSH), Molecular Sequence Data (MeSH), Mutant Proteins (genetics), Mutant Proteins (immunology), Mutation, Missense (MeSH), RNA, Viral (genetics), Sequence Analysis, DNA (MeSH), Spain (MeSH).
- MESH :
- chemical , genetics : Antigens, Viral, Epitopes, Hemagglutinin Glycoproteins, Influenza Virus, Mutant Proteins, RNA, Viral.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Antigens, Viral, Epitopes, Hemagglutinin Glycoproteins, Influenza Virus, Mutant Proteins.
- geographic : Spain.
- genetics : Influenza A Virus, H1N1 Subtype.
- immunology : Influenza A Virus, H1N1 Subtype.
- isolation & purification : Influenza A Virus, H1N1 Subtype.
- virology : Influenza, Human.
- Animals, Humans, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Mutation, Missense, Sequence Analysis, DNA.
Abstract
Murine hybridomas producing neutralizing mAbs specific to the pandemic influenza virus A/California/07/2009 haemagglutinin (HA) were isolated. These antibodies recognized at least two different but overlapping new epitopes that were conserved in the HA of most Spanish pandemic isolates. However, one of these isolates (A/Extremadura/RR6530/2010) lacked reactivity with the mAbs and carried two unique mutations in the HA head (S88Y and K136N) that were required simultaneously to eliminate reactivity with the murine antibodies. This unusual requirement directly illustrates the phenomenon of enhanced antigenic change proposed previously for the accumulation of simultaneous amino acid substitutions at antigenic sites of the influenza A virus HA during virus evolution (Shih et al., Proc Natl Acad Sci USA, 104 , 6283-6288, 2007). The changes found in the A/Extremadura/RR6530/2010 HA were not found in escape mutants selected in vitro with one of the mAbs, which contained instead nearby single amino acid changes in the HA head. Thus, either single or double point mutations may similarly alter epitopes of the new antigenic site identified in this work in the 2009 H1N1 pandemic virus HA. Moreover, this site is relevant for the human antibody response, as shown by competition of mAbs and human post-infection sera for virus binding. The results are discussed in the context of the HA antigenic structure and challenges posed for identification of sequence changes with possible antigenic impact during virus surveillance.
DOI: 10.1099/vir.0.061598-0
PubMed: 24531414
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Delgado, Teresa" sort="Delgado, Teresa" uniqKey="Delgado T" first="Teresa" last="Delgado">Teresa Delgado</name><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Benito, Sonia" sort="Benito, Sonia" uniqKey="Benito S" first="Sonia" last="Benito">Sonia Benito</name><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Casas, Inmaculada" sort="Casas, Inmaculada" uniqKey="Casas I" first="Inmaculada" last="Casas">Inmaculada Casas</name><affiliation wicri:level="3"><nlm:affiliation>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Pozo, Francisco" sort="Pozo, Francisco" uniqKey="Pozo F" first="Francisco" last="Pozo">Francisco Pozo</name><affiliation wicri:level="3"><nlm:affiliation>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Cuevas, Maria Teresa" sort="Cuevas, Maria Teresa" uniqKey="Cuevas M" first="María Teresa" last="Cuevas">María Teresa Cuevas</name><affiliation wicri:level="3"><nlm:affiliation>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Mas, Vicente" sort="Mas, Vicente" uniqKey="Mas V" first="Vicente" last="Mas">Vicente Mas</name><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Trento, Alfonsina" sort="Trento, Alfonsina" uniqKey="Trento A" first="Alfonsina" last="Trento">Alfonsina Trento</name><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Rodriguez Frandsen, Ariel" sort="Rodriguez Frandsen, Ariel" uniqKey="Rodriguez Frandsen A" first="Ariel" last="Rodriguez-Frandsen">Ariel Rodriguez-Frandsen</name><affiliation wicri:level="3"><nlm:affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation></author><author><name sortKey="Falc N, Ana" sort="Falc N, Ana" uniqKey="Falc N A" first="Ana" last="Falc N">Ana Falc N</name><affiliation wicri:level="3"><nlm:affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation></author><author><name sortKey="Ortin, Juan" sort="Ortin, Juan" uniqKey="Ortin J" first="Juan" last="Ortín">Juan Ortín</name><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Nieto, Amelia" sort="Nieto, Amelia" uniqKey="Nieto A" first="Amelia" last="Nieto">Amelia Nieto</name><affiliation wicri:level="3"><nlm:affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation></author><author><name sortKey="Melero, Jose A" sort="Melero, Jose A" uniqKey="Melero J" first="José A" last="Melero">José A. Melero</name><affiliation wicri:level="1"><nlm:affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares</wicri:regionArea><wicri:noRegion>Illes Baleares</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="eISSN">1465-2099</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Antigens, Viral (genetics)</term><term>Antigens, Viral (immunology)</term><term>Epitopes (genetics)</term><term>Epitopes (immunology)</term><term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term><term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term><term>Humans (MeSH)</term><term>Influenza A Virus, H1N1 Subtype (genetics)</term><term>Influenza A Virus, H1N1 Subtype (immunology)</term><term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term><term>Influenza, Human (virology)</term><term>Mice (MeSH)</term><term>Mice, Inbred BALB C (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Mutant Proteins (genetics)</term><term>Mutant Proteins (immunology)</term><term>Mutation, Missense (MeSH)</term><term>RNA, Viral (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Spain (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Animaux (MeSH)</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes viraux (génétique)</term><term>Antigènes viraux (immunologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Espagne (MeSH)</term><term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term><term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term><term>Grippe humaine (virologie)</term><term>Humains (MeSH)</term><term>Mutation faux-sens (MeSH)</term><term>Protéines mutantes (génétique)</term><term>Protéines mutantes (immunologie)</term><term>Souris (MeSH)</term><term>Souris de lignée BALB C (MeSH)</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (immunologie)</term><term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term><term>Épitopes (génétique)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term><term>Epitopes</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>Mutant Proteins</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Antigens, Viral</term><term>Epitopes</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>Mutant Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Spain</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Antigènes viraux</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Protéines mutantes</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Antigènes viraux</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Protéines mutantes</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Mutation, Missense</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Espagne</term><term>Humains</term><term>Mutation faux-sens</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Espagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Murine hybridomas producing neutralizing mAbs specific to the pandemic influenza virus A/California/07/2009 haemagglutinin (HA) were isolated. These antibodies recognized at least two different but overlapping new epitopes that were conserved in the HA of most Spanish pandemic isolates. However, one of these isolates (A/Extremadura/RR6530/2010) lacked reactivity with the mAbs and carried two unique mutations in the HA head (S88Y and K136N) that were required simultaneously to eliminate reactivity with the murine antibodies. This unusual requirement directly illustrates the phenomenon of enhanced antigenic change proposed previously for the accumulation of simultaneous amino acid substitutions at antigenic sites of the influenza A virus HA during virus evolution (Shih et al., Proc Natl Acad Sci USA, 104 , 6283-6288, 2007). The changes found in the A/Extremadura/RR6530/2010 HA were not found in escape mutants selected in vitro with one of the mAbs, which contained instead nearby single amino acid changes in the HA head. Thus, either single or double point mutations may similarly alter epitopes of the new antigenic site identified in this work in the 2009 H1N1 pandemic virus HA. Moreover, this site is relevant for the human antibody response, as shown by competition of mAbs and human post-infection sera for virus binding. The results are discussed in the context of the HA antigenic structure and challenges posed for identification of sequence changes with possible antigenic impact during virus surveillance. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24531414</PMID><DateCompleted><Year>2014</Year><Month>06</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1465-2099</ISSN><JournalIssue CitedMedium="Internet"><Volume>95</Volume><Issue>Pt 5</Issue><PubDate><Year>2014</Year><Month>May</Month></PubDate></JournalIssue><Title>The Journal of general virology</Title><ISOAbbreviation>J. Gen. Virol.</ISOAbbreviation></Journal><ArticleTitle>Characterization of an enhanced antigenic change in the pandemic 2009 H1N1 influenza virus haemagglutinin.</ArticleTitle><Pagination><MedlinePgn>1033-1042</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/vir.0.061598-0</ELocationID><Abstract><AbstractText>Murine hybridomas producing neutralizing mAbs specific to the pandemic influenza virus A/California/07/2009 haemagglutinin (HA) were isolated. These antibodies recognized at least two different but overlapping new epitopes that were conserved in the HA of most Spanish pandemic isolates. However, one of these isolates (A/Extremadura/RR6530/2010) lacked reactivity with the mAbs and carried two unique mutations in the HA head (S88Y and K136N) that were required simultaneously to eliminate reactivity with the murine antibodies. This unusual requirement directly illustrates the phenomenon of enhanced antigenic change proposed previously for the accumulation of simultaneous amino acid substitutions at antigenic sites of the influenza A virus HA during virus evolution (Shih et al., Proc Natl Acad Sci USA, 104 , 6283-6288, 2007). The changes found in the A/Extremadura/RR6530/2010 HA were not found in escape mutants selected in vitro with one of the mAbs, which contained instead nearby single amino acid changes in the HA head. Thus, either single or double point mutations may similarly alter epitopes of the new antigenic site identified in this work in the 2009 H1N1 pandemic virus HA. Moreover, this site is relevant for the human antibody response, as shown by competition of mAbs and human post-infection sera for virus binding. The results are discussed in the context of the HA antigenic structure and challenges posed for identification of sequence changes with possible antigenic impact during virus surveillance. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>García-Barreno</LastName><ForeName>Blanca</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Delgado</LastName><ForeName>Teresa</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Benito</LastName><ForeName>Sonia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Casas</LastName><ForeName>Inmaculada</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pozo</LastName><ForeName>Francisco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cuevas</LastName><ForeName>María Teresa</ForeName><Initials>MT</Initials><AffiliationInfo><Affiliation>National Influenza Centre at Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Virus Respiratorios & Gripe, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mas</LastName><ForeName>Vicente</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Trento</LastName><ForeName>Alfonsina</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodriguez-Frandsen</LastName><ForeName>Ariel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Falcón</LastName><ForeName>Ana</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ortín</LastName><ForeName>Juan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nieto</LastName><ForeName>Amelia</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Centro Nacional de Biotecnología, CSIC, Cantoblanco, Madrid, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Melero</LastName><ForeName>José A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>CIBER de Enfermedades Respiratorias, Mallorca, Illes Baleares, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>KF860844</AccessionNumber><AccessionNumber>KF860845</AccessionNumber><AccessionNumber>KF860846</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>02</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Gen Virol</MedlineTA><NlmUniqueID>0077340</NlmUniqueID><ISSNLinking>0022-1317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000939">Epitopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019267">Hemagglutinin Glycoproteins, Influenza Virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D050505">Mutant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000956" MajorTopicYN="N">Antigens, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000939" MajorTopicYN="N">Epitopes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019267" MajorTopicYN="N">Hemagglutinin Glycoproteins, Influenza Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053118" MajorTopicYN="N">Influenza A Virus, H1N1 Subtype</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050505" MajorTopicYN="N">Mutant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020125" MajorTopicYN="N">Mutation, Missense</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013030" MajorTopicYN="N" Type="Geographic">Spain</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24531414</ArticleId><ArticleId IdType="doi">10.1099/vir.0.061598-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><country name="Espagne"><noRegion><name sortKey="Garcia Barreno, Blanca" sort="Garcia Barreno, Blanca" uniqKey="Garcia Barreno B" first="Blanca" last="García-Barreno">Blanca García-Barreno</name></noRegion><name sortKey="Benito, Sonia" sort="Benito, Sonia" uniqKey="Benito S" first="Sonia" last="Benito">Sonia Benito</name><name sortKey="Benito, Sonia" sort="Benito, Sonia" uniqKey="Benito S" first="Sonia" last="Benito">Sonia Benito</name><name sortKey="Casas, Inmaculada" sort="Casas, Inmaculada" uniqKey="Casas I" first="Inmaculada" last="Casas">Inmaculada Casas</name><name sortKey="Casas, Inmaculada" sort="Casas, Inmaculada" uniqKey="Casas I" first="Inmaculada" last="Casas">Inmaculada Casas</name><name sortKey="Cuevas, Maria Teresa" sort="Cuevas, Maria Teresa" uniqKey="Cuevas M" first="María Teresa" last="Cuevas">María Teresa Cuevas</name><name sortKey="Cuevas, Maria Teresa" sort="Cuevas, Maria Teresa" uniqKey="Cuevas M" first="María Teresa" last="Cuevas">María Teresa Cuevas</name><name sortKey="Delgado, Teresa" sort="Delgado, Teresa" uniqKey="Delgado T" first="Teresa" last="Delgado">Teresa Delgado</name><name sortKey="Delgado, Teresa" sort="Delgado, Teresa" uniqKey="Delgado T" first="Teresa" last="Delgado">Teresa Delgado</name><name sortKey="Falc N, Ana" sort="Falc N, Ana" uniqKey="Falc N A" first="Ana" last="Falc N">Ana Falc N</name><name sortKey="Falc N, Ana" sort="Falc N, Ana" uniqKey="Falc N A" first="Ana" last="Falc N">Ana Falc N</name><name sortKey="Garcia Barreno, Blanca" sort="Garcia Barreno, Blanca" uniqKey="Garcia Barreno B" first="Blanca" last="García-Barreno">Blanca García-Barreno</name><name sortKey="Mas, Vicente" sort="Mas, Vicente" uniqKey="Mas V" first="Vicente" last="Mas">Vicente Mas</name><name sortKey="Mas, Vicente" sort="Mas, Vicente" uniqKey="Mas V" first="Vicente" last="Mas">Vicente Mas</name><name sortKey="Melero, Jose A" sort="Melero, Jose A" uniqKey="Melero J" first="José A" last="Melero">José A. Melero</name><name sortKey="Melero, Jose A" sort="Melero, Jose A" uniqKey="Melero J" first="José A" last="Melero">José A. Melero</name><name sortKey="Nieto, Amelia" sort="Nieto, Amelia" uniqKey="Nieto A" first="Amelia" last="Nieto">Amelia Nieto</name><name sortKey="Nieto, Amelia" sort="Nieto, Amelia" uniqKey="Nieto A" first="Amelia" last="Nieto">Amelia Nieto</name><name sortKey="Ortin, Juan" sort="Ortin, Juan" uniqKey="Ortin J" first="Juan" last="Ortín">Juan Ortín</name><name sortKey="Ortin, Juan" sort="Ortin, Juan" uniqKey="Ortin J" first="Juan" last="Ortín">Juan Ortín</name><name sortKey="Pozo, Francisco" sort="Pozo, Francisco" uniqKey="Pozo F" first="Francisco" last="Pozo">Francisco Pozo</name><name sortKey="Pozo, Francisco" sort="Pozo, Francisco" uniqKey="Pozo F" first="Francisco" last="Pozo">Francisco Pozo</name><name sortKey="Rodriguez Frandsen, Ariel" sort="Rodriguez Frandsen, Ariel" uniqKey="Rodriguez Frandsen A" first="Ariel" last="Rodriguez-Frandsen">Ariel Rodriguez-Frandsen</name><name sortKey="Rodriguez Frandsen, Ariel" sort="Rodriguez Frandsen, Ariel" uniqKey="Rodriguez Frandsen A" first="Ariel" last="Rodriguez-Frandsen">Ariel Rodriguez-Frandsen</name><name sortKey="Trento, Alfonsina" sort="Trento, Alfonsina" uniqKey="Trento A" first="Alfonsina" last="Trento">Alfonsina Trento</name><name sortKey="Trento, Alfonsina" sort="Trento, Alfonsina" uniqKey="Trento A" first="Alfonsina" last="Trento">Alfonsina Trento</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Characterization of an enhanced antigenic change in the pandemic 2009 H1N1 influenza virus haemagglutinin.
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